HPLC does have very low sensitivity for particular compounds, and several can not be detected as These are irreversibly adsorbed.Also, it has a higher sensitivity though a reduced sample consumption. HPLC has one particular edge more than GC column that analysis can be done for any sample might be stably dissolved while in the eluent and need never
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The main parts of a HPLC are demonstrated in Determine (PageIndex 3 ). The role of the pump is always to force a liquid (cell stage) through at a selected circulation price (milliliters for every minute). The injector serves to introduce the liquid sample into the move stream on the mobile section. Column is the most central and critical element of